SCIENTIFIC RESEARCHER

Employment: 

Research laboratory at Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove

Working position: 

Academic and research staff

Education and qualification:

2015 - 2020 

Charles University, Faculty of Pharmacy in Hradec Králové, doctoral study program Bioanalytical chemistry

dissertation thesis: The application of HPLC and UHPLC-MS/MS techniques in clinical research

supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D.

co-supervior: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D.

2010 - 2015 

Charles University, Faculty of Pharmacy in Hradec Králové, study program Pharmacy

diploma thesis: Development of HPLC method for determination of vancomycin in clinical research

2002 - 2010 

Secondary school - Secondary Grammar School, Český Těšín

Work Experience:

2019 - 2026

Researcher, Research laboratory at Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic 

2015 - 2019

Teaching, selected subjects at the Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University

2015 - 2020

Postgraduate student, Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University

2015 

Present junior researcher, Research laboratory of Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové

Courses:

2018 

19. School of mass spectrometry

2017 

18. School of mass spectrometry

2016 

Mass spectrometry in organic analysis

Total publications: 28, H-Index 6, Sum of cit.: 362, Sum of IF: 81.4

(update 6/2025)

Publications:

2026

28. Hrubsa, M., Paclikova, M., Konecny, L., Matousova, K., Kujovska Krcmova L., Carazo, A., Smahelova, A., Blaha, V., Mladenka, P., A comparison of the antiplatelet effects of acetylsalicylic acid and a small dietary phenolic metabolite, 4-methylcatechol, in type 2 diabetes mellitus patients, 2026, in preparation

27. Hrubsa, M., Konecny, L., Sirova, M., Homolkova, S., Carazo, A., Matousova, K., Kujovska Krcmova, L., Bernhauerova, V., Blaha, V., Smahelova, A., Mladenka, P., Platelet reactivity in polymorbid older patients and the effect of 4-methycatechol, BMC Geriatrics, 2026, DOI: 10.1186/s12877-026-07945-2

26. Privratska, N., Matous, P., Erben, J., Matousova, K., Satinsky, D., Svec, F., Kujovska Krcmova, L., Centrifugation-assisted extraction for rapid retinol analysis in human serum using functionalized glass beads, Advances in Sample Preparation, 2026, 19, 100239, DOI: 10.1016/j.sampre.2026.100239

25. Krupa, P., Kujovska Krcmova, L., Krupova, M., Matousova, K., Paulikova, S., Kasparova, P., Melichar, B., Cesak, T., Neopterin, Tryptophan and Kynurenine as Biomarkers in Glioblastoma, Scientific Reports, 2026, DOI: 10.1038/s41598-026-52612-6 

2025

24. Gunaseelan, C., Lomozova, Z., Hrubsa, M., Paclikova, M., Skorepa, M., Matousova, K., Turonova, D., Kujovska Krcmova, L., Blaha, M., Blaha, V., Mladenka, P., Carazo, A., Occurrence of Platelet-Monocyte Aggregates in Patients with Metabolic Disorders and Effect of Direct Anticoagulants, Thrombosis and Haemostasis, 2025, DOI: 10.1055/a-2747-8963

23. Paclikova, M., Konecny, L., Carazo, A., Matousova, K., Kujovska Krcmova, L., Blaha, V., Smahelova, A., Mladenka, P., Acetylsalicylic acid and vorapaxar are less active, while 4-methylcatechol is more active, in type 1 diabetic patients compared to healthy controls, Cardiovascular Diabetology, 2025, 24(1): 323, DOI: 10.1186/s12933-025-02891-6

22. Carazo, A., Paclikova, M., Fadraersada, J., Alva-Galleos, R., Skorepa, P., Gunaseelan, C., Matousova, K., Mrstna, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Mladenka, P., Type 1 diabetes mellitus patients had lower total vitamin K levels and increased sensitivity to direct anticoagulants, PLoS One, 2025, 20(6): e0326580, DOI: 10.1371/journal.pone.0326580

21. Kujovska Krcmova, L., Matousova, K., Javorska, L., Mrstna, K., Privratska, N., Suwanvecho, Ch., Turonova, D., Farrag, M., Kacerovsky, M. Melichar, B., Urinary kynurenine, tryptophan and neopterin concentrations during physiological pregnancy, Scientific Reports, 2025, 15(1): 19674, DOI: 10.1038/S41598-025-04642-9

20. Siatka, T., Matus, M., Moravcova, M., Harcarova, P., Lomozova, Z., Matousova, K., Suwanvecho, Ch., Kujovska Krcmova, L., Mladenka, P., Biological, dietetic and pharmacological properties of vitamin B9, npj Science of Food, 2025, 9, 30, DOI: 10.1038/s41538-025-00396-w

19. Fadraersada, J., Alva‑Gallegos, R., Skorepa, P., Musil, F., Javorska, L., Matousova, K., Kujovska Krcmova, L., Paclikova, M., Carazo, A., Blaha M., Blaha, V., Mladenka, P., Coaugulation in familial hypercholesterolemic patients: effect of current hypolipidemic treatment and anticoagulants, Naunyn-Schmiedeberg's Archives of Pharmacology, 2025, 398(6), 7343-7352, DOI:10.1007/s00210-024-03740-1

2024

18. Moravcova, M., Siatka, T., Kujovska Krcmova, L., Matousova, K., Mladenka, P., Biological properties of vitamin B12, Nutrition Research Reviews, 2024, 1-33, DOI: 10.1017/S0954422424000210

17. Carazo, A., Hrubsa, M., Konecny, L., Gunaseelan, C., Fadraersada, J., Skorepa, P., Paclikova, M., Musil, F., Karlickova, J., Javorska, L., Matousova, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Mladenka, P., Correlations among different platelet aggregation pathways in a group of healthy volunteers, Platelets, 2024, 35(1), DOI: 10.1080/09537104.2024.2336093

16. Kujovska Krcmova, L., Javorska, L., Matousova, K., Smahel, P., Skala, M., Kopecky, M., Suwanvecho, Ch., Privratska, N., Turonova, D., Melichar, B., Evaluation of inflammatory biomarkers and vitamins in hospitalized patients with SARS-CoV-2 infection and post-COVID syndrome, Clinical Chemistry and Laboratory Medicine, 2024, 62(6), DOI: 10.1515/cclm-2023-1297

15. Fadraersada, J., Alva‑Gallegos, R., Skorepa, P., Musil, F., Javorska, L., Matousova, K., Kujovska Krcmova, L., Paclikova, M., Carazo, A., Blaha, V., Mladenka, P., Head‑to‑head ex vivo comparison of clinically used direct anticoagulant drugs, Naunyn-Schmiedeberg's Archives of Pharmacology, 2024, 397(6), DOI: 10.1007/s00210-023-02891-x

14. Mrstna, K., Matousova, K., Kujovska Krcmova, L., Carazo, A., Pourova, J., Mladenka, P., Matysova, L., Svec, F., Analysis of vitamin K1 and major K2 variants in rat/human serum and lipoprotein fractions by a rapid, simple, and sensitive UHPLC-MS/MS method, Journal of Chromatography A, 2024, 464548, DOI: 10.1016/j.chroma.2023.464548

2023

13. Chocholous, P., Matousova, K., Satinsky, D., Kujovska Krcmova, L., Sklenarova, H., Longevity and other practical benefits of monolithic silica columns in the analysis of samples with complex matrices, Journal of Separation Science, 2023, 2300448, DOI: 10.1002/jssc.202300448

12. Kujovska Krcmova, L., Matousova, K., Javorska, L., Smahel, P., Skala, M., Koblizek, V., Skop, J., Turonova, D., Gancarcikova, M., Melichar, B., Neopterin and kynurenice in serum and urine as prognostic biomarkers in hospitalized patients with delta and omicron variant SARS-CoV-2 infection, Clinical Chemistry and Laboratory Medicine, 2023, 61(11), 2053-2064, DOI: 10.1515/cclm-2023-0381

11. Konecny, L., Hrubsa, M., Karlickova, Carazo, A., Javorska, L., Matousova, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Blaha, M., Mladenka, P., The Impact of Convertase Subtilisin/Kexin Type 9 Monoclonal Antibodies with and without Apheresis on Platelet Aggregation in Familial Hypercholesterolemia, Cardiovascular Drugs and Therapy, 2023, DOI: 10.1007/s10557-023-07455-y

10. Konecny, L., Hrubsa, M., Karlickova, J., Carazo, A., Javorska, L., Matousova, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Blaha, M., Mladenka, P., The Effect of 4-Methylcatechol on Platelets in Familial Hypercholesterolemic Patients Treated with Lipid Apheresis and/or Proprotein Convertase Subtilisin Kexin 9 Monoclonal Antibodies, Nutrients, 2023, 15, 1842, DOI: 10.3390/nu15081842

9. Spisarova, M., Melichar, B., Juranova, J., Zemankova, A., Adam, T., Matousova, K., Javorska, L., Kujovska Krcmova, L., Turonova, D., Studentova, H., Biomarkers of Inflammation and Progression During Immunotherapy in Patients with Metastatic Renal Cell Carcinoma, In Vivo, 2023, 37, 393-399, DOI: 10.21873/invivo.13091

8. Mrstna, K., Matousova, K., Matous, P., Matysova, L., Svec, F., Snejdrova, E., Kujovska Krcmova, L., Analysis of terbinafine in PLGA-based drug delivery systems by a fast and sensitive UHPLC-DAD method, Analytical Methods, 2023, 15, 2823-2832, DOI: 10.1039/D3AY00506B

2022

7. Hrubsa, M., Konecny, L., Paclikova, M., Parvin, S., Skorepa, P., Musil, F., Karlickova, J., Javorska, L., Matousova, K., Kujovska Krcmova, L., Carazo, A., Smahelova, A., Blaha, V., Mladenka, P., The Antiplatelet Effect of 4-Methylcatechol in a Real Population Sample and Determination of the Mechanism of Action, Nutrients, 2022, 14. DOI: 10.3390/xxxx

6. Carazo, A., Hrubsa, M., Konecny, L., Skorepa, P., Paclikova, M., Musil, F., Karlickova, J., Javorska, L., Matousova, K., Kujovska Krcmova, L., Parvin, S., Smahelova, A., Blaha, V., Mladenka, P., Sex-Related Differences in Platelet Aggregation: A Literature Review Supplemented with Local Data from a Group of Generally Healthy Individuals, Seminars in Thrombosis & Hemostasis, 2022, DOI: 10.1055/s-0042-1756703

5. Hrubsa, M., Siatka, T., Nejmanova, I., Voprsalova, M., Kujovska Krcmova, L., Matousova, K., Javorska, L., Macakova, K., Mercolini, L., Remião, F., Maťus, M., Mladenka, P., Biological properties of vitamins of the B-complex, part 1 – vitamins B1, B2, B3 and B5, Nutrients, 2022, 14, 484

2021

4. Kaska, M., Havel, E., Javorska, L., Matousova, K., Paral, J., Chobola, M., Safranek, P., Bezouska, J., Kujovska Krcmova, L., Can early postoperative parenteral nutrition have some impact on postoperative inflammatory response intensity?, Clinical Nutrition Espen, 2021, 45, p. 150-154. DOI: 10.1016/j.clnesp.2021.07.022

3. Carazo, A., Macakova, K., Matousova, K., Kujovska Krcmova, L., Protti, M., Mladenka, P., Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity, Nutrients, 2021, 13(5), 1703, DOI: 10.3390/nu13051703

2019

2. Kucerova K., Kujovska Krcmova L., Mikanova Z., Matysova L., Melichar B., Svec F., Determination of urinary retinol and kreatinin as an early sensitive marker of renul dysfunction, Journal of Chromatography A, 2019, 1607, a. n. 460390

1. Kucerova K., Reiska, V., Svec, F., Kujovska Krcmova, L., Matysova, L., Fast determination of omeprazole in extemporaneous suspensions used in paediatrics and stability studies, Analytical Methods, 2019, 11 (4), 517-523, DOI: 10.1039/c8ay02547a

Grant Projects:

2025 - 2028

Ministry of Education Youths and Sports of the Czech Republic - project CZ.02.01.01/00/23_020/0008535

OPERATIONAL PROGRAMME JOHANNES AMOS COMENIUS

- Advanced techniques for biomedical diagnostics (ATEBIO)

- A co-investigator

2025 - 2027

Ministry of Health of the Czech Republic - project NW24-05-00031 (AZV 9037)

- Non infectious manifestations and dysregulation of the immune system in primary antibody immunodeficiencies - a multidisciplinary approach to diagnosis, risk assessment and treatment

- A co-investigator

2021 - 2022

Ministry of Health of the Czech Republic - project NU21J-02-00021 (AZV 9028)

- Differences in parameters of platelets aggregation and blood coaugulation between healthy individuals and patients with metabolic diesases

- A co-investigator

Internships:

2019

Faculty of Chemistry, Jagiellonian University in Krakow (1 month)