LEADER

Associate Professor RNDr. Lenka KUJOVSKÁ KRČMOVÁ, Ph.D.


Employment: 

Research laboratory at Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove and Department of Analytical Chemistry, Faculty of Pharmacy, Charles University in Prague

Working position: 

Academic and research staff


Education and qualification:

2020 - present

Member of the panel P07 Age-specific group diseases of the Czech Health Research Council of the Czech Republic

2018

Member of rigorous committee of Analytical chemistry in Faculty of Pharmacy Charles University, Czech Republic

Member of council for subject Bioanalytical methods n Faculty of Pharmacy Charles University, Czech Republic 

7.7. 2017 - 30.8. 2019

Member of accreditation and examination committee of specialized education Laboratory investigated methods in health care (by law no.95/2004 CR)

1. 2. 2017

Associate Professor of Analytical Chemistry, Faculty of Pharmacy in Hradec Kralove, Charles University In Prague. Thesis: " Development of chromatographic methods for clinical research "

10. - 12. 2013

University of Tasmania - research fellowship (Hobart, Tasmania, Australia)

11.5. 2011

RNDr. Graduation

2010

Dissertation work: "Development and validation of new HPLC methods for determination of biologically active compounds in clinical practice" - Ph.D. graduation

2007 and 2008

Erasmus and Erasmus Practical Placement study at School of Chemical Sciences, Dublin City University, Dublin, Ireland (3 and 4 months)

2006 - 2010

Ph.D. study at Department of Analytical Chemistry, Faculty of Pharmacy, Charles University in Prague, at external work place at Research laboratory of 3rd Internal Gerontometabolic Clinic, University Hospital Hradec Kralove

2001 - 2006

Study and graduation at Faculty of Pharmacy, Charles University in Prague, specialization: Medical Bioanalysis, Diploma thesis: "Development and validation of HPLC method for determination of retinyl esters in human serum using monolithic column"


Awards:

2023

The BEST POSTER PRESENTATION on the 13th International Conference on Instrumental Methods of Analysis: Modern Trends and Applications in Greece (IMA 2023), the theme "Microsampling and modern sample preparation technigues in bioanalysis for clinical research". - 20.9.2023

2019

Medal Faculty of Pharmacy in Hradec Králové, Charles University

2014

Selected by Academy of Science of the Czech Republic for participation in 65th Lindau Nobel Laureate Meeting (2015 Interdisciplinary)

Invited main speaker in 9th International Conference of Anticancer Research (Sithonia, Greece) - 6.-10.10.2014

2012

Member of Charles University Research Centre (UNCE 204026/2012) Study of Drugs and Other Biologically-Active Substances Perspective in Prevention and Treatment of Important Civilization Diseases

2011

Third place French embassy and Sanofi Aventis Pharmacy Award 2011 - 16.6.2011

Hradec Kralove Mayor Award nomination (in section student creative work) - 31.3.2011

Bolzano Award (University Award) in medical scientist category, Project: "Development and validation of new HPLC methods for determination of biologically active compounds in clinical practice" - 24.2.2011

2010

Josef Hlávka Award (prestige award for the best students and absolvents of Prague universities, the Academy of Sciences of the Czech Republic and University of Technology in Brno) - 17.11.2010

2008

First place - oral presentation (till age 35) at conference 6th Aegean Analytical Chemistry Days, Denizli, Turkey - 12.10.2008


Teaching:

Faculty of Pharmacy, Charles University in Prague (subjects - laboratory practice): Analytical Chemistry, Methods of Instrumental Analysis, Separation Methods, Food Analysis, Special methods of instrumental analysis

Supervision of students: 

Supervision - bachelor and diploma thesis (13), rigorosis projects (1), summer practice students (30), postgradual students (7)


Total publications: 105, H-Index 19, Sum of IF= 331.016, Sum of cit.: 1070, Sum of cit. with. self-citation: 855

(update 1/2024)

PUBLICATIONS in last 5 years (2020-2024): 31

First author/Corresponding/Last author: 16

More than 50 posters and oral presentations at home and international conferences.

Publications:

2024

105. Carazo, A., Hrubsa, M., Konecny, L., Gunaseelan, C., Fadraersada, J., Skorepa, P., Paclikova, M., Musil, F., Karlickova, J., Javorska, L., Matousova, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Mladenka, P., Correlations among different platelet aggregation pathways in a group of healthy volunteers, Platelets, 2024, In Press, DOI: 10.1080/09537104.2024.2336093

104. Mrstna, K., Turonova, D., Suwanvecho, Ch., Svec, F., Kujovska Krcmova, L., The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum, Microchemical Journal, 2024, 198, 110170, DOI: 10.1016/j.microc.2024.110170  

103. Kujovska Krcmova, L., Javorska, L., Matousova, K., Smahel, P., Skala, M., Kopecky, M., Suwanvecho, Ch., Privratska, N., Turonova, D., Melichar, B., Evaluation of inflammatory biomarkers and vitamins in hospitalized patients with SARS-CoV-2 infection and post-COVID syndrome, Clinical Chemistry and Laboratory Medicine, 2024, DOI: 10.1515/cclm-2023-1297

102. Zibridova, K., Soucek, O., Kujovska Krcmova, L., Jankovicova, K., Gancarcikova, M., Pejkova, MA., Drugda, J., Novakova, D., Kostal, M., Lymphocyte subpopulations: a potential predictor of a response in patients with immune thrombocytopenia, Hematology, 2024, 29(1), 2304486, DOI: 10.1080/16078454.2024.2304486

101. Fadraersada, J., Alva‑Gallegos, R., Skorepa, P., Musil, F., Javorska, L., Matousova, K., Kujovska Krcmova, L., Paclikova, M., Carazo, A., Blaha, V., Mladenka, P., Head‑to‑head ex vivo comparison of clinically used direct anticoagulant drugs, Naunyn-Schmiedeberg's Archives of Pharmacology, 2024, In Press, DOI: 10.1007/s00210-023-02891-x

100. Mrstna, K., Matousova, K., Kujovska Krcmova, L., Carazo, A., Pourova, J., Mladenka, P., Matysova, L., Svec, F., Analysis of vitamin K1 and major K2 variants in rat/human serum and lipoprotein fractions by a rapid, simple, and sensitive UHPLC-MS/MS method, Journal of Chromatography A, 2024, 464548, DOI: 10.1016/j.chroma.2023.464548

2023

99. Turonova, D., Kujovska Krcmova, L., Vosmik, M., Melichar, B., Svec, F., Using HPLC for the determination of platinum drugs in biological matrixes after derivatization with diethyldithiocarbamate, Journal of Separation Science, 2023, 46(19): e2300392, DOI: 10.1002/jssc.202300392 

98. Chocholous, P., Matousova, K., Satinsky, D., Kujovska Krcmova, L., Sklenarova, H., Longevity and other practical benefits of monolithic silica columns in the analysis of samples with complex matrices, Journal of Separation Science, 2023, 2300448, DOI: 10.1002/jssc.202300448 

97. Mrstna, K., Kujovska Krcmova, L., Svec, F., Advances in kynurenine analysis, Clinica Chimica Acta, 2023, 547, 117441, DOI: 10.1016/j.cca.2023.117441 

96. Kujovska Krcmova, L., Matousova, K., Javorska, L., Smahel, P., Skala, M., Koblizek, V., Skop, J., Turonova, D., Gancarcikova, M., Melichar, B., Neopterin and kynurenice in serum and urine as prognostic biomarkers in hospitalized patients with delta and omicron variant SARS-CoV-2 infection, Clinical Chemistry and Laboratory Medicine, 2023, 61(11), 2053-2064, DOI: 10.1515/cclm-2023-0381 

95. Suwanvecho, Ch., Kujovska Krcmova, L., Svec, F., Effective, convenient, and green sample preparation for the determination of retinol and retinol acetate in human serum using pipette tip microextraction, Talanta, 2023, 262, 124689, DOI: 10.1016/j.talanta.2023.124689 

94. Konecny, L., Hrubsa, M., Karlickova, Carazo, A., Javorska, L., Matousova, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Blaha, M., Mladenka, P., The Impact of Convertase Subtilisin/Kexin Type 9 Monoclonal Antibodies with and without Apheresis on Platelet Aggregation in Familial Hypercholesterolemia, Cardiovascular Drugs and Therapy, 2023, DOI: 10.1007/s10557-023-07455-y 

93. Konecny, L., Hrubsa, M., Karlickova, J., Carazo, A., Javorska, L., Matousova, K., Kujovska Krcmova, L., Smahelova, A., Blaha, V., Blaha, M., Mladenka, P., The Effect of 4-Methylcatechol on Platelets in Familial Hypercholesterolemic Patients Treated with Lipid Apheresis and/or Proprotein Convertase Subtilisin Kexin 9 Monoclonal Antibodies, Nutrients, 2023, 15, 1842, DOI: 10.3390/nu15081842 

92. Spisarova, M., Melichar, B., Juranova, J., Zemankova, A., Adam, T., Matousova, K., Javorska, L., Kujovska Krcmova, L., Turonova, D., Studentova, H., Biomarkers of Inflammation and Progression During Immunotherapy in Patients with Metastatic Renal Cell Carcinoma, In Vivo, 2023, 37, 393-399, DOI: 10.21873/invivo.13091 

91. Mrstna, K., Matousova, K., Matous, P., Matysova, L., Svec, F., Snejdrova, E., Kujovska Krcmova, L., Analysis of terbinafine in PLGA-based drug delivery systems by a fast and sensitive UHPLC-DAD method, Analytical Methods, 2023, 15, 2823-2832, DOI: 10.1039/D3AY00506B 

2022

90. Heneberk, O., Vernerova, A., Kujovska Krcmova, L., Wurfelova, E., Radochova, V., Neopterin levels in periodontitis and after nonsurgical periodontal therapy: Evaluation of gingival crevicular fluid, oral fluid, serum and urinary samples - A case control study, Biomedicines, 2022, 10, 3200. DOI: 10.3390/biomedicines10123200 

89. Hrubsa, M., Konecny, L., Paclikova, M., Parvin, S., Skorepa, P., Musil, F., Karlickova, J., Javorska, L., Matousova, K., Kujovska Krcmova, L., Carazo, A., Smahelova, A., Blaha, V., Mladenka, P., The Antiplatelet Effect of 4-Methylcatechol in a Real Population Sample and Determination of the Mechanism of Action, Nutrients, 2022, 14. DOI: 10.3390/xxxx 

88. Carazo, A., Hrubsa, M., Konecny, L., Skorepa, P., Paclikova, M., Musil, F., Karlickova, J., Javorska, L., Matousova, K., Kujovska Krcmova, L., Parvidn, S., Smahelova, A., Blaha, V., Mladenka, P., Sex-Related Differences in Platelet Aggregation: A Literature Review Supplemented with Local Data from a Group of Generally Healthy Individuals, Seminars in Thrombosis & Hemostasis, 2022, DOI: 10.1055/s-0042-1756703 

87. Vernerova, A., Kujovska Krcmova, L., Heneberk, O., Radochova, V., Svec, F., Liquid chromatography method with tandem mass spectrometry and fluorescence detection for determination of inflammatory biomarkers in gingival crevicular fluid as a tool for diagnosis of periodontal disease, Journal of Pharmaceutical and Biomedical Analysis, 2022, 212, a. n. 114644. DOI: 10.1016/j.jpba.2022.114644 

86. Hrubsa, M., Siatka, T., Nejmanova, I., Voprsalova, M., Kujovska Krcmova, L., Matousova, K., Javorska, L., Macakova, K., Mercolini, L., Remião, F., Maťus, M., Mladenka, P., Biological properties of vitamins of the B-complex, part 1 – vitamins B1, B2, B3 and B5, Nutrients, 2022, 14, 484 

2021

85. Vernerova, A., Kujovska Krcmova, L., Melichar, B., Svec, F., Non-invasive determination of uric acid in human saliva in the diagnosis of serious disorders, Clinical Chemistry and Laboratory Medicine, 2021, 59 (5), p. 797-812. DOI: 10.1515/cclm-2020-1533 

84. Kaska, M., Havel, E., Javorska, L., Matousova, K., Paral, J., Chobola, M., Safranek, P., Bezouska, J., Kujovska Krcmova, L., Can early postoperative parenteral nutrition have some impact on postoperative inflammatory response intensity?, Clinical Nutrition Espen, 2021, 45, p. 150-154. DOI: 10.1016/j.clnesp.2021.07.022 

83. Vernerova, A., Garcia-Souza, L.F., Soucek, O., Kostal, M., Rehacek, V., Kujovska Krcmova, L., Gnaiger, E., Sobotka, O., Mitochondrial Respiration of Platelets: Comparison of Isolation Methods, Biomedicines, 2021, 9 (12), a. n. 1859. DOI: 10.3390/biomedicines9121859 

82. Mladenka, P., Macakova, K., Kujovska Krcmova, L., Javorska, L., Mrstna, K., Carazo, A., Protti, M., Remiao, F., Novakova, L., Vitamin K- sources, physiological role, kinetics, deficiency, detection, therapeutic use and toxicity, Nutrition Reviews, 2021, 80(4), 677-698, DOI: 10.1093/nutrit/nuab061 

81. Turonova, D., Kujovska Krcmova, L., Svec, F., Application of microextraction in pipette tips in clinical and forensic toxicology, Trac-trends In Analytical Chemistry, 2021, 143, 116404. 

80. Vernerova, A., Kujovska Krcmova, L., Heneberk, O., Radochova, V., Strouhal, O., Kasparovsky, A., Melichar, B., Svec, F. Chromatographic method for the determination of inflamantory biomarkers and uric acid in human saliva, Talanta, 2021, 233, 122598. 

79. Carazo, A., Macakova, K., Matousova, K., Kujovska Krcmova, L., Protti, M., Mladenka, P., Vitamin A Update: Forms, Sources, Kinetics, Detection, Function, Deficiency, Therapeutic Use and Toxicity, Nutrients, 2021, 13(5), 1703. 

78. Dosedel, M., Jirkovsky, D., Macakova, K., Kujovska Krcmova, L., Javorska, L., Pourova, J., Mercolini, L., Remião, F., Novákova, L., Mladenka, P., Vitamin C-Sources, Physiological Role, Kinetics, Deficiency, Use, Toxicity, and Determination, Nutrients, 2021, 13(2), 615, 2-34. 

2020

77. Kujovska Krcmova, L., Melichar, B., Svec, F., Chromatographic methods development for clinical practice: requirements and limitations, Clinical Chemistry And Laboratory Medicine, 2020, 58(11), 1785-1793 

76. Aydogan, C., Rigano, F., Kujovska Krcmova, L., Chung, D. S., Macka, M., Mondello, L., Miniaturized LC in Molecular Omics, Analytical Chemistry, 2020, 92(17), 11485-11497 

75. Jenco J., Kujovska Krcmova L., Sobotka O., Blaha V. Solich P., Development of novel liquid chromatography method for clinical monitoring of vitamin B1 metabolites and B6 status in the whole blood, Talanta, 2020, 211, a. n. 120702 

2019

74. Kucerova K., Kujovska Krcmova L., Mikanova Z., Matysova L., Melichar B., Svec F., Determination of urinary retinol and kreatinin as an early sensitive marker of renul dysfunction, Journal of Chromatography A, 2019, 1607, a. n. 460390 

73. Kucerova K., Reiska V., Svec F., Kujovska Krcmova L., Matysova L., Fast determination of omeprazole in extemporaneous suspensions used in paediatrics and stability studies, Analytical Methods, 2019, 11 (4), 517-523 

2018

72. Pilka R., Neubert D., Stejskal D., Krejci G., Svestak M., Marek R., Adam T., Sobkova K., Ondrova D., Hambalek J., Maderka M., Solichova D., Kujovska Krcmova L., Javorska L., Melichar B., Serum concentrations of TFF3, S100-A11 and AIF-1 in association with systemic inflammatory response, dinase stage and nodal involvement in endometrial cancer, Pteridines, 2018, 29 (1), 6-12 

71. Dusek T., Orhalmi J., Sotona O., Kujovska Krcmova L., Javorska L., Dolejs J., Paral J., Neopterin, kynurenine and tryptofan as a new biomarkers for early detection of rectal anastomotic leakage, Videosurgery And Other Miniinvasive Techniques, 2018, 13 (1), 44-52 

2017

70. Mergancova J., Javorska L., Siller J., Sakra L., Mergancova J., Solichova D., Kujovska Krcmova L., Melichar B., Mohelnikova-Duchonova B., Studentova H., Klos D., Concentrations of neopterin, kynurenine and tryptofan in wound secretions of patients with breast cancer and malignant melanoma: a pilot study, Pteridines, 2017, 28 (3-4), 205-211 

69. Melichar B., Vitaskova D., Bartouskova M., Javorska L., Kujovska Krcmova L., Peskova E., Hyspler R., Solichova D., Hruzova K., Studentova H., Comparison of performance of composite biomarkers of inflammatory response in determining the prognosis of breast cancer patients, Pteridines, 2017, 28 (3-4), 213-220 

68. Melichar B., Hruzova K., Kujovska Krcmova L., Javorska L., Peskova E., Solichova D., Hyspler R., Malirova E., Vosmik M., Bartouskova M., Klos D., Studentova H., Association of peripheral blood cell count-derived ratios, biomarkers of inlfammatory response and tumor growth with outcome in previously treated metastatic colorectal carcinoma patients receiving cetuximab, Pteridines, 2017, 28 (3-4), 221-232 

67. Vitaskova D., Melichar B., Bartouskova M., Vlachova Z., Vrana D., Jankova J., Adam T., Juranova J., Zlamalova N., Kujovska Krcmova L., Javorska L., Klos D., Studentova H., Neoadjuvant combination therapy with trastuzumab in a breast cancer patient with synchronous rectal carcinoma: a case report and biomarker study, Pteridines, 2017, 28 (3-4), 233-241 

66. Blaha V., Solichova D., Kujovska Krcmova L., Lanska M., Havel E., Vyroubal P., Zadak Z., Zak P., Sobotka L., Antioxidant defense systém in familial hypercholesterolemia and the effects of lipoprotein apheresis, Atherosclerosis Supplements, 2017, 30, 159-165 

65. Kucerova K., Kujovska Krcmova L., Matysova L., Solich P., Could urinary retinol be used as a new biomarker of kidney damage?, Trac-trends In Analytical Chemistry, 2017, 95, 57-61 

64. Cervinkova B., Kujovska Krcmova L., Klabackova S., Solichova D., Solich P., Rapid determination of lipophilic vitamins in human serum by ultra-high performance liquid chromatography using a fluorinated throughput miniaturized liquid-liquid extraction, Journal Of Separation Science, 2017, 40 (17), 3375-3382 

63. Jenco J., Kujovska Krcmova L., Solichova D., Solich P., Recent trends in determinativ of thiamine and its derivatives in clinical practice, Journal Of Chromatography A, 2017, 1510, 1-12 

62. Javorska L., Kujovska Krcmova L., Solich P., Kaska M., Simple and rapid quantification of vancomycin in serum, urine and peritoneal/pleural effusion via UHPLC-MS/MS applicable to personalized antibiotik dosing, Journal Of Pharmaceutical And Biomedical Analysis, 2017, 142, 59-65 

61. Melichar B., Spisarova M., Bartouskova M., Kujovska Krcmova L., Javorska L., Studentova H. Neopterin as a biomarker of imine response in cancer patiens, Annals Of Translational Medicine, 2017, 5 (13), a. n. 280 

60. Cervinkova B., Kujovska Krcmova L., Sestakova V., Solichova D., Solich P. A fully validated bioanalytical method using an UHPLC-MS/MS systém for quantification of DNA and RNA oxidative stress biomarkers, Analytical And Bioanalytical Chemistry, 2017, 409 (14), 3611-3621 

59. Blaha V., Blaha M., Lanska M., Solichova D., Kujovska Krcmova L., Havel E., Vyroubal P., Zadak Z., Zak P., Sobotka L., Lipoprotein Apheresis in the Treatment of Dyslipidemia – the Czech Republic Experience, Physiological Research, 2017, 66, S91-S100 

2016

58. Melichar B., Studentova H., Vitaskova D., Sramek V., Kujovska Krcmova L., Peskov E., Solichova D., Kalabova H., Ryska A., Hruzova K., Havlik R., Association of urinary neopterin, neutrolil-to-lymphocyte, lymfocyte-to-monocyte and platelet-to-lymphocyte ratios with long-term survival of patiens with breast cancer, Pteridines, 2016, 27 (3-4), 59-65 

57. Kralickova P., Vokurkova D., Haviger J., Rezacova V., Solichova D., Kujovska Krcmova L., Melichar B., Havlik R., Peripheral blood leukocyte populations and urinary neopterin during chemotherapy in patiens with breast cancer, Pteridines, 2016, 27 (3-4), 67-75 

56. Pilka R., Marek R., Adam T., Kudela M., Ondrova D., Neubert D., Hambalek J., Maderka M., Solichova D., Kujovska Krcmova L., Melichar B., Systematic inflammatory Response After open , Laparoscopic and Robotic Surgery in Endometrial Cancer Patients, Anticancer Research, 2016, 36 (6), 2909-2922 

55. Vonka V., Petrackova M., Humlova Z., Klamova H., Kujovska Krcmova L., Duskova M., Prochazka B., The relationship of Kynurenine and Neopterin Levels and Their Association with a Selectionof Other Immune Markers in Chronic Myeloid Leukaemia Patients, Folia Biologica, 2016, 62 (6), 235-240 

54. Cervinkova B., Kujovska Krcmova L., Solichova D., Melichar B., Solich P., Recent advances in the determination of tocopherols in biological fluids: from sample pre treatment and liquid chromatography to clinical studies, Analytical And Bioanalytical Chemistry, 2016, 408 (10), 2407-2424 

53. Javorska L., Kujovska Krcmova L., Solichova D., Solich P., Kaska M., Modern methods for vancomycin determination in biological fluids by HPLC based methods – A review, Journal Of Separation Science, 2016, 39 (1), 6-20 

52. Zezulova M., Bartouskova M., Hlidkova E., Adam T., Kujovska Krcmova L., Cervinkova B., Solichova D., Zlevorova M., Cwiertka K., Friedecky D., Vrana D., Melichar B., Citrulline as a biomarker of gastrointestinal toxicity in patients with rectal carcinoma treated with chemoradiation, Clinical Chemistry And Laboratory Medicine, 2016, 54 (2), 305-314. 

51. Zezulova M., Bartouskova M., Hlidkova E., Juranova J., Cervinkova B., Kasalova E., Adam T., Kujovska Krcmova L., Solichova D., Cwiertka K., Vrana D., Melichar B., Prognostic Significance of Serum and Urinary Neopterin Concentrations in Patients with Rectal Carcinoma Treated with Chemoradiation, Anticancer Research, 2016, 35(1), 287-292. 

2015

50. Kujovska Krcmova L., Cervinkova B., Solichova D., Sobotka L., Hansmanova L., Melichar B., Solich P., Fast and sensitive HPLC method for the determination of neopterin, kynurenine and tryptophan in amniotic fluid, malignant effusions and wound exudates, Bioanalysis, 2015, 7 (21), 2751-2762 

49. Studentova H., Vitaskova D., Sramek V., Indrakova J., Adam T., Juranova J., Petrova P., Kujovska Krcmova L., Peskova E., Solichova D., Kalabova H., Melichar B., Correlations of neutrophil-to-lymphocyte, lymphocyte-to-monocyte and platelet-to-lymphocyte ratios with biomarkers of atherosclerosis risk and inflammatory response in patients with a history of breast cancer, Pteridines, 2015, 26(4), 161-172. 

48. Yan L., Dvorak M., Nesterenko P., Stanley R., Nuchtavorn N., Kujovska Krcmova L., Aufartova J., Macka M., Miniaturised medium pressure capillary liquid chromatography system with flexible open platform design using off- the-shelf microfluidic components. Analytica Chimica Acta, 2015, 896, 177-176. 

47. Aufartova J., Blaha M., Kasalova E., Honegrova B., Cervinkova B., Kujovska Krcmova L., Plisek J., Lanska M., Sobotka L., Solichova D., Blood levels of antioxidant agents during age-related macular degeneration treatment by rheohaemapheresis, Biomedical Papers-Olomouc, 2015, 159 (3), 400-406 

46. Vonka V., Humlova Z., Klamova H., Kujovska Krcmova L., Petrackova M., Hamsikova E., Krmenickova-Fliegl M., Dusková M., Roth Z., Kynurenine and uric acid levels in chronic myeloid leukemia patients, Oncoimmunology, 2015, 4 (3), a. n. e992646 

45. Solichova D., Blaha M., Aufartova J., Kujovska Krcmova L., Plisek J., Honegrova B., Kasalova E., Lanska M., Urbanek L., Sobotka L., The effect of LDL-apheresis and rheohaemapheresis treatment on vitamin E, Journal Of Nutritional Science And Vitaminology, 2015, 61 (2), 105-112 

44. Plisek J., Pospichalova N., Khalikova M., Aufartova J., Solichova D., Kujovska Krcmova L., Solich P., A combination of ultracentrifugation and solid-phase extraction with subsequent chromatographic analysis of alpha tocopherol in erythrocyte membranes, Journal Of Separation Science, 2015, 34, 634-640 

43. Kasalova, E., Aufartova, J., Kujovska Krcmova, L., Solichova, D., Solich, P., Recent trends in the analysis of vitamin D and its metabolites in milk – A review, Food Chemistry 2015, 171, 177-190 

2014

42. Melichar, B., Kalabova, H., Kujovska Krcmova, L., Trivedi, S., Kralickova, P., Malirova, E., Pecka, M., Studentova, H., Zezulova, M., Holeckova, P., Solichova, D., Urinary Neopterin Concentrations During Combination Therapy with Cetuximab in Previously Treated Patients with Metastatic Colorectal Carcinoma, In Vivo, 2014, 28(5), 953-959 

41. Petru, K., Siroka, J., Bydzovska, L., Kujovska Krcmova, L., Polasek, M., Assay of urinary 8-hydroxy-2 '-deoxyguanosine by capillary electrophoresis with spectrophotometric detection using a high-sensitivity detection cell and solid-phase extraction, Electrophoresis, 2014, 35(17), 2546-2549 

40. Melichar, B., Dolezal, B., Sramek, V., Kalabova, H., Kujovska Krcmova, L., Hyspler, R., Studentova, H., Vosmik, M., Pecka, M., Svobodnik, A., Pecen, L., Dolezel, M., Solichova, D., Prevalence of Perfusion Defects Detected by Stress (99m)Technetium Sestamibi Myocardial Perfusion Single-photon Emission Computed Tomography in Asymptomatic Patients with Breast Cancer, Anticancer Research, 2014, 34(7), 3689-3694 

39. Trivedi, S.V., Melichar, B., Myjirsky, M., Vesely, P., Kalabova, H., Studentova, H., Kujovska Krcmova, L., Solichova, D., Dolezel, M., Urinary neopterin concentrations during radiotherapy for gynecological cancer, Pteridines, 2014, 25(1), 1-5 

2013

38. Plisek, J., Kujovska Krcmova, L., Aufartova, J., Morales, T.V., Esponda, S.M., Oros, R., Kasalova, E., Santana.Rodriguez, J.J., Sobotka, L., Solich, P., Solichova, D., New approach for the clinical monitoring of 25-hydroxyvitamin D-3 and 25-hydroxyvitamin D-2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972, Journal Of Separation Science, 2013, 36(23), 3702-3708 

37. Sramek, V., Melichar, B., Indrakova, J., Studentova, H., Kalabova, H., Vrana, D., Lukesova, L., Adam, T., Hlidkova, E., Juranova, J., Petrova, P., Kujovska Krcmova, L., Solichova, D., Risk factors of atherosclerosis in patients with history of breast cancer, Pteridines, 2013, 24(3-4), 201-210 

36. Sramek, V., Melichar, B., Studentova, H., Kalabova, H., Vrana, D., Lukesova, L., Adam, T., Hlidkova, E., Juranova, J., Kujovska Krcmova, L., Solichova, D., Systemic immune response and peripheral blood cell count in patients with a history of breast cancer, Pteridines, 2013, 24(3-4), 211-217, 210 

35. Krcmova, L., Solichova, D., Solich, P., Microplates in liquid chromatography - New solution in clinical research? - A review, Talanta, 2013, 115, 973-979 

34. Holeckova, P., Krcmova, L., Letal, J., Svobodnik, A., Kalabova, H., Kasparova, M., Plisek, J., Pala, M., Vitek, P., Solichova, D., Zezulova, M., Studentova, H., Dolezel, M., Melichar, B., Urinary Neopterin Concentration and Toxicity of Radiotherapy in Patients with Head and Neck Carcinoma During External Beam Radiation, Anticancer Research, 2013, 33(9), 4097-4101 

33. Kucerova, B., Krcmova, L., Solichova, D., Plisek, J., Solich, P., Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and alpha-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography, Journal Of Separation Science, 2013, 36(14), 2223-2230 

32. Plisek, J., Kasparova, M., Solichova, D., Krcmova, L., Kucerova, B., Sobotka, L., Solich, P., Application of core-shell technology for determination of retinol and alpha-tocopherol in breast milk, Talanta, 2013, 107, 382-388 

2012

31. Melichar, B., Kalabova, H., Ungermann, L., Krcmova, L., Hyspler, R., Kasparova, M., Pecka, M., Sramek, V., Prochazkova-Studentova, H., Svobodnik, A., Pecen, L., Solichova, D., Carotid Intima-media Thickness and Laboratory Parameters of Atherosclerosis Risk in Patients with Breast Cancer, Anticancer Research, 2012, 32(9), 4077-4084 

30. Kasparova, M., Plisek, J., Solichova, D., Krcmova, L., Kucerova, B., Hronek, M., Solich, P., Rapid sample preparation procedure for determination of retinol and alpha-tocopherol in human breast milk, Talanta, 2012, 93, 147-152 

29. Holeckova, P., Krcmova, L., Kalabova, H., Kasparova, M., Plisek, J., Pala, M., Vitek, P., Solichova, D., Zezulova, M., Studentova, H., Melichar, B., Prognostic Significance of Serum Retinol, Serum Alpha-Tocopherol, and Urinary Neopterin in Patients with Head and Neck Carcinoma Treated with External Beam Radiation, International Journal For Vitamin And Nutrition Research, 2012, 82(2), 77-84 

28. Cincibuch, J., Cwiertka, K., Prochazkova-Studentova, H., Zezulova, M., Neoral, C., Kalabova, H., Krcmova, L., Malirova, E., Solichova, D., Melichar, B., Prognostic Significance of Serum Neopterin in Patients with Esophageal Carcinoma, Pteridines, 2012, 23(1), 22-26 

2011

27. Kalabova, H., Melichar, B., Ungermann, L., Dolezal, J., Krcmova, L., Kasparova, M., Plisek, J., Hyspler, R., Pecka, M., Solichova, D., Intima-media thickness, myocardial perfusion and laboratory risk factors of atherosclerosis in patients with breast cancer treated with anthracycline-based chemotherapy, Medical Oncology, 2011, 28(4), 1281-1287 

26. Krcmova, L., Solichova, D., Melichar, B., Kasparova, M., Plisek, J., Sobotka, L., Solich, P., Determination of neopterin, kynurenine, tryptophan and creatinine in human serum by high throuput HPLC, Talanta, 2011, 85(3), 1466-1471 

25. Melichar, B., Kalabova, H., Krcmova, L., Kasparova, H., Plisek, J., Cermna, J., Holeckova, P., Solichova, D., Urinary Neopterin in Patients with Metastatic Colon Cancer Treated with Patupilone, Pteridines, 2011, 22(3), 61-65 

24. Kralickova, P., Kalabova, H., Krcmova, L., Kasparova, M., Plisek, J., Ungermann, L., Vokurkova, D., Hyspler, R., Pecka, M., Sramek, V., Studentova, H., Zezulova, M., Solichova, D., Melichar, B., Correlation of Peripheral Blood CD14(+)CD16(+)Monocytes, Urinary Neopterin and the Risk Factors of Atherosclerosis in Patients with Breast Carcinoma, Pteridines, 2011, 22(3), 66-72 

23. Sklenarova, H., Koblova, P., Chocholous, P., Satinsky, D., Krcmova, L., Kasparova, M., Solichova, D., Solich, P., Separation of Vitamins Retinol Acetate, Ergocalciferol, or Cholecalciferol and Tocopherol Acetate Using Sequential Injection Chromatography, Analytical Letters, 2011, 44(1-3), 446-456 

22. Kalabova, H., Krcmova, L., Kasparova, M., Plisek, J., Laco, J., Hyspler, R., Klozova, H., Solichova, D., Melichar, B., Prognostic significance of increased urinary neopterin concentrations in patients with breast carcinoma, European Journal of Gynaecological Oncology, 2011, 32(5), 525-529 

2010

21. Melichar, B., Kasparova, M., Kalabova, H., Dvorak, J., Hyspler, R., Ticha, A., Krcmova, L., Plisek, J., Holeckova, P., Solichova, D., Intestinal Permeability, Vitamin A Absorption and Serum Alpha-Tocopherol in Gastrointestinal Stromal Tumor Patients Treated with Imatinib, Journal Of Nutritional Science And Vitaminilogy, 2010, 56(6), 347-352 

20. Melichar, B., Kalabova, H., Krcmova, L., Kasparova, M., Plisek, J., Hyspler, R., Studentova, H., Zezulova, M., Holeckova, P., Pecka, M., Solichova, D., Urinary Neopterin, Serum Retinol, alpha-tocopherol and Homocysteine in Breast Cancer Patients During Treatment with Bevacizumab and Chemotherapy, Pteridines, 2010, 21(4), 87-93 

19. Dvorak, J., Melichar, B., Hyspler, R., Krcmova, L., Urbanek, L., Kalabova, H., Kasparova, M., Solichova, D., Intestinal permeability, vitamin A absorption, alpha-tocopherol, and neopterin in patients with rectal carcinoma treated with chemoradiation, Medical Oncology, 2010, 27(3), 690-696 

18. Melichar, B., Krcmova, L., Kalabova, H., Holeckova, P., Kasparova, M., Plisek, J., Hyspler, R., Studentova, H., Solichova, D., Serum Retinol, Alpha-Tocopherol and Systemic Inflammatory Response in Metastatic Colorectal Carcinoma Patients Treated with Combination Chemotherapy and Cetuximab, Journal Of Nutritional Science And Vitaminology, 2010, 56(4), 222-226 

17.  Melicharova, K., Kalabova, H., Krcmova, L., Urbanek, L., Solichova, D., Melichar, B., Effect of comorbidity on urinary neopterin in patients with breast carcinoma, European Journal Of Cancer Care, 2010, 19(3), 340-345 

16. Melichar, B., Dvorak, J., Kalabova, H., Hyspler, R., Krcmova, L., Kasparova, M., Urbanek, L., Solichova, D., Intestinal permeability, vitamin A absorption and serum alpha-tocopherol during therapy with gefitinib, Scandinavian Journal Of Clinical Laboratory & Investigation, 2010, 70(3), 180-187 

15. Melichar, B., Plisek, J., Hyspler, R., Kalabova, H., Kasparova, M., Krcmova, L., Studentova, H., Solichova, D., Erythrocyte Alpha-Tocopherol in Breast Cancer Patients Treated with Letrozol, Journal Of Nutritional Science And Vitaminology, 2010, 56(2), 98-103 

14. Krcmova, L., Solichova, D., Plisek, J., Kasparova, M., Sobotka, L., Solich, P., Miniaturisation of solid phase extraction method for determination of retinol, alpha- and gamma-tocopherol in human serum using new technologies, International Journal Of Enviromental Analytical Chemistry, 2010, 90(2), 106-114 

2009

13. Melichar, B., Kalabova, H., Krcmova, L., Kasparova, M., Malirova, E., Melicharova, K., Pecka, M., Hyspler, R., Solichova, D., Serum Homocysteine, Cholesterol, Retinol, alpha-Tocopherol, Glycosylated Hemoglobin and Inflammatory Response during Therapy with Bevacizumab, Oxaliplatin, 5-Fluorouracil and Leucovorin, Anticancer Research, 2009, 29(11), 4813-4820 

12. Melichar, B., Kalabova, H., Krcmova, L., Urbanek, L., Hyspler, R., Solichova, D., Melicharova, K., Pecka, M., Zadak, Z., Effect of Aromatase Inhibitors on Lipid Metabolism, Inflammatory Response and Antioxidant Balance in Patients with Breast Carcinoma, Anticancer Research, 2009, 29(8), 3337-3346 

11. Krcmova, L., Urbanek, L., Solichova, D., Kasparova, M., Vlckova, H., Melichar, B., Sobotka, L., Solich, P., HPLC method for simultaneous determination of retinoids and tocopherols in human serum for monitoring of anticancer therapy, Journal Of Separation Science, 2009, 32(15-16), 2804-2811 

10. Cincibuch, J., Melichar, B., Studentova, H., Kapustova, M., Malirova, E., Solichova, D., Krcmova, L., Kasparova, M., Schneiderka, P., Juranova, J., Sramek, V., Cwiertka, K., Serum Neopterin, Retinol and Alpha-tocopherol in Patients with the Carcinoma of the Esophagus, Pteridines, 2009, 20(2), 49-53 

9. Krcmova, L., Stjernof, A., Mehlen, S., Hauser, P.C., Abele, S., Paull, B., Macka, M., Deep-UV-LEDs in photometric detection: A 255 nm LED on-capillary detector in capillary electrophoresis, Analyst, 2009, 134(12), 2394-2396 

2008

8. Melichar, B., Dvorak, J., Krcmova, L., Hyspler, R., Urbanek, L., Solichova, D., Intestinal Permeability and Vitamin A Absorption in Patients With Chemotherapy-induced Diarrhea, American Journal of Clinical Oncology-Cancer Clinical Trials, 2008, 31(6), 580-584 

7. Melichar, B., Ballokova, A., Malirova, E., Urbanek, L., Krcmova, L., Hyspler, R., Hornychova, H., Ryska, A., Solichova, D., Circulating Vascular Endothelial Growth Factor, C-reactive Protein and Urinary Neopterin Concentrations During dose-dense Chemotherapy, Pteridines, 2008, 19(3), 65-71 

6. Melichar, B., Urbanek, L., Krcmova, L., Kalabova, H., Melicharopva, K., Malirova, E., Hornychova, H., Ryska, A., Hyspler, R., Solichova, D., Urinary neopterin, hemoglobin and peripheral blood cell counts in breast carcinoma patients treated with dose-dense chemotherapy, Anticancer Research, 2008, 28(4C), 2389-2396 

5. Melichar, B., Hyspler, R., Kalabova, H., Urbanek, L., Krcmova, L., Solichova, D., Gastrointestinal permeability - a parameter of possible prognostic importance in metastatic colorectal carcinoma, Pteridines, 2008, 19(1), 19-22 

2007

4. Melichar, B., Kalabova, H., Krcmova, L., Urbanek, L., Hyspler, R., Malirova, E., Solichova, D., Serum alpha-tocopherol, retinol and neopterin during paclitaxel/carboplatin chemotherapy, Anticancer Research, 2007, 27(6C), 4397-4401 

3. Kalabova, H., Dvorak, J., Hyspler, R., Ticha, A., Krcmova, L., Urbanek, L., Solichova, D., Melichar, B., Urinary neopterin in patients treated with gefitinib, Pteridines, 2007, 18(3), 95-100 

2006

2. Melichar, B., Urbanek, L., Krcmova, L., Kalabova, H., Svobodova, I., Dragounova, E., Vesely, P., Hyspler, R., Solichova, D., Urinary neopterin in patients with ovarian cancer, Pteridines, 2006, 17(4), 145-153 

1. Urbanek, L., Krcmova, L., Solichova, D., Melichar, B., Opletalova, V., Solich, P., Development and validation of a liquid chromatography method for the simultaneous determination of alpha-tocopherol, retinol and retinyl esters in human serum using a monolithic column for the monitoring of anticancer therapy side effects, Journal Of Separation Science, 2006, 29(16), 2485-2493


Grants:

Principal investigator: 7 projects, NU22-A-108 (2022-2023), IGA Ministry of Health grant NT/14265 (2013-2015) - (A evaluation), GAUK 88407/2008-2009, FRVŠ 374/2008, FRVŠ 973/2009, 2017-2023 - University Hospital Internal grants HK 00179906

Co investigator: 12 projects, Ministry of Health grants IGA NT/13564-4/2012 (2012-2015), NT/13566-4/2012 (2012-2015), IGA NT14089-3/2013 (2012-2015), GAUK 263811/2011 (2011-2013, FRVŠ 121/2013 (2013), Research project of Faculty of Pharmacy Hradec Kralove 140/57/55705 (2015), Ministry of Health grants AZV-VES 17-31754A (2017-2020), AZV-VES 17-29241A (2017-2020), AZV-VES 17-28882A (2017-2020), AZV-VES NV18-03-00130 (2018-2021), European union grant CZ.02.1.01/0.0/0.0/17_048/0007441 PERSONMED (2018-2022)

Participation on grants: 5 projects, Ministry of Health grant IGA NR/9096-4 (2005-2009), IGA NR/9096-4 (2005-2009), IGA NR/9259-3 (2007-2009), Research project of University Hospital Hradec Kralove MZO 00179906 (2005-2011), Research project of Faculty of Pharmacy Hradec Kralove MSM 0021620822 (2005-2011)

Member of Charles University Research Centre (UNCE 204026/2012) Study of Drugs and Other Biologically-Active Substances Perspective in Prevention and Treatment of Important Civilization Diseases (2012 - 2017) 


CHROMED
Chromatography Method Development
 Bioanalytical Research

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