SCIENTIFIC RESEARCHER

Employment: 

Research laboratory at Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Kralove

Working position: 

Research staff

Education and qualification:

2024

RNDr. graduation 

2023

Ph.D. graduation 

2018 - 2023

Charles University, Faculty of Pharmacy, Hradec Králové, doctoral study program Bioanalytical Methods

Dissertation thesis: Development of new methods for clinical research using LC-MS techniques 

Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D.

2016 - 2018 

Charles University, Faculty of Pharmacy, Hradec Králové, master study program Specialist in Laboratory Methods

2013 - 2016 

Charles University, Faculty of Pharmacy, Hradec Králové, bachelor study program Medical Laboratory Technician

2022 

University of Bologna-Alma mater studiorum (Bologna, Italy), Department of Pharmacy and Biotechnology, Research Group of Pharmaco-Toxicological Analysis (PTA Lab)

2018 

University of Seville (Spain), Faculty of Pharmacy, Department of Analytical Chemistry - internship- 3 months
- Olive oil analysis-analysis of volatile compounds, quality analysis

2017 

University of Seville (Spain), Faculty of Pharmacy, Department of Nutrition and Bromatology, Toxicology, and Legal Medicine - internship- 2 months

Work Experience:

2023 - 2024

Researcher, Research laboratory at Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic

2018 - 2023 

An assistant of practical training, Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic

2018 - present 

A junior researcher, Research laboratory at Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic

2016 

Department of Biological Analyses, Public Health Institute, Ostrava, Czech Republic
- water analysis, food analysis - summer job

Courses:

2021 

Czech Chromatographic School - HPLC 2021

2020 

21st School of mass spectrometry

2019 

Czech Chromatographic school - HPLC 2019

Mass spectrometry in organic analysis (course at University of Pardubice)20th School of mass spectrometry

Total publications: 6

Publications:

2024

6. Mrstna, K., Turonova, D., Suwanvecho, Ch., Svec, F., Kujovska Krcmova, L., The power of modern extraction techniques: A breakthrough in vitamin K extraction from human serum, Microchemical Journal, 2024, 198, 110170, DOI: 10.1016/j.microc.2024.110170   

5. Kujovska Krcmova, L., Javorska, L., Matousova, K., Smahel, P., Skala, M., Kopecky, M., Suwanvecho, Ch., Privratska, N., Turonova, D., Melichar, B., Evaluation of inflammatory biomarkers and vitamins in hospitalized patients with SARS-CoV-2 infection and post-COVID syndrome, Clinical Chemistry and Laboratory Medicine, 2024, DOI: 10.1515/cclm-2023-1297

2023

4. Turonova, D., Kujovska Krcmova, L., Vosmik, M., Melichar, B., Svec, F., Using HPLC for the determination of platinum drugs in biological matrixes after derivatization with diethyldithiocarbamate, Journal of Separation Science, 2023, 46(19), e2300392, DOI: 10.1002/jssc.202300392

3. Kujovska Krcmova, L., Matousova, K., Javorska, L., Smahel, P., Skala, M., Koblizek, V., Skop, J., Turonova, D., Gancarcikova, M., Melichar, B., Neopterin and kynurenice in serum and urine as prognostic biomarkers in hospitalized patients with delta and omicron variant SARS-CoV-2 infection, Clinical Chemistry and Laboratory Medicine, 2023, 61(11), 2053-2064, DOI: 10.1515/cclm-2023-0381

2. Spisarova, M., Melichar, B., Juranova, J., Zemankova, A., Adam, T., Matousova, K., Javorska, L., Kujovska Krcmova, L., Turonova, D., Studentova, H., Biomarkers of Inflammation and Progression During Immunotherapy in Patients with Metastatic Renal Cell Carcinoma, In Vivo, 2023, 37, 393-399, DOI: 10.21873/invivo.13091

2021 

1. Turonova, D., Kujovska Krcmova, L., Svec, F., Application of microextraction in pipette tips in clinical and forensic toxicology, Trac-trends In Analytical Chemistry, 2021, 143, 116404. DOI: 10.1016/j.trac.2021.116404

Grant Projects:

2023 - 2024

Ministry of Health of the Czech Republic - project NU21J-02-00021

- Differences in parameters of platelets aggregation and blood coaugulation between healthy individuals and patients with metabolic diesases

- A co-investigator

2021 - 2022 

The Charles University Grant Agency Project
- Modern preanalysis as a tool for solving complications in the determination of vitamin K
- A co-author of the grant

2021 

University Hospital Research Grant 81-28
- Determination of individual forms of vitamin K using a combination of modern separation techniques UHPLC-MS / MS and ultracentrifugation for future testing of differences in blood coagulation parameters between healthy persons and patients with metabolic diseases
- A member of the research team

2018 - 2021 

Ministry of Health of the Czech Republic - project NV 18-03-00130
- Specific factors affecting the toxicity of hyperthermic intraperitoneal chemotherapy
- A member of the research team since 2019

Conferences:

2024

3rd European Sample Preparation Conference - EuSP 2024, Chania, Greece

2nd Green and Sustainable Analytical Chemistry Conference - GSAC 2024, Chania, Greece

2023

12th Aegean Analytical Chemistry Days, Istanbul, Turkey

51st International Symposium of High Performance Liquid Phase Separation and Related Techniques, Düsseldorf, Germany

2022 

26th International Symposium on Separation Sciences, Ljubljana, Slovenia
"Evaluation of a New Microfluidic DBS Technology for Cocaine Analysis"
Dorota Turonova, Marco Cirrincione, Lenka Kujovska Krcmova, Roberto Mandrioli, Michele Protti, Laura Mercolini

8th EBF Young Scientist Symposium, Hasselt, Belgium
" Microsampling for cocaine analysis - the future or just a fairy tale?" (Oral presentation)
Dorota Turonova, Lenka Kujovska Krcmova, Michele Protti, Laura Mercolini

12th Postgraduate and Postdoc Conference, Hradec Kralove, Czech Republic
"Application of derivatization in analysis of platinum compounds" (Oral presentation) - awarded by scientific committee for excellent oral presentation

2021 

5th International Caparica Christmas Conference on Sample Treatment 2021, Caparica, Portugal
"Challenges in sample preparation for cytostatic toxicity evaluation in various matrices after HIPEC" (Poster contribution) Dorota Turonova, Lenka Kujovska Krcmova
-awarded with Excellent Poster Presentation Prize
"A modern UHPLC-MS/MS method for the determination of vitamin K in serum/plasma" (Poster contribution)
Kristýna Mrstna, Dorota Turonova, Lenka Kujovska Krcmova, Ludmila Matysova 

Czech Chromatographic School - HPLC 2021, Zaječí, Czech Republic
"Challenges and pitfalls of in-situ derivatization in bioanalysis" (Poster contribution) Dorota Turonová, Lenka Kujovska Krcmova 

11th Postgraduate and Postdoc Conference, Hradec Králové, Czech Republic
"Determination of platinum drugs in human plasma after hyperthermic intraperitoneal chemotherapy" (Oral presentation)

2020 

10th Postgraduate and Postdoc Conference, Hradec Králové, Czech Republic
"The method development for the determination of cisplatin in human plasma" (Oral presentation)

Awards:

2023

20.9.2023

The POSTER PRESENTATION - 2nd place on the 12th Aegean Analytical Chemistry Days in Turkey (AACD 2023), the theme "Neopterin, kynurenine and tryptophan as a new prognostic markers in hospitalized patients with SARS-CoV-2 infection".

Languages:

Czech - native speaker

English - C1

Polish - C1

Spanish- A2

Italian- A2

Other skills:

MS Office, LabSolutions software, Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS) software